Tobacco whitefly (bemisia tabaci) ke kokoanyana e kotsi e ajoang lefatšeng ka bophara 'me e ka ba le phello e matla tlhahisong ea meroho. Ho hanana le tshireletso ya dijalo ke bo bong ba mathata a tshebetsong. Ka hona, ho lemoha liphetoho tsa liphatsa tsa lefutso ho ka fana ka tšupiso ea bohlokoa bakeng sa taolo ea likokoanyana. Leha ho le joalo, tabaci ea Bemisia e nyenyane, e bolelele ba 'mele bo ka tlaase ho 1mm. Ts'ebetso e tloaelehileng ea ho latellana ha hlooho e le 'ngoe e thata,' me hangata ho fumanoa palo e nyenyane ea gDNA, empa e ja nako e ngata le lisebelisoa, 'me mekhoa e mecha ea ho lemoha e hlokahala ka potlako lipatlisisong tsa saense.
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The Vegetable Pest Research Laboratory e thehile mokhoa oa ho lemoha maqhubu a phetoho ea liphatsa tsa lefutso ka har'a likokoanyana tse nyane tse sebelisang theknoloji ea amplicon le ho lemoha maqhubu a liphetoho tse peli tsa pyrethroid tse amanang le ho hanyetsa liphatsa tsa lefutso tsa sodium ion channel sechabeng sa Bemisia tabaci. Mokhoa ona o sebetsa hantle ebile o ka tšeptjoa mme o rarolla bothata ba ho lemoha maqhubu a phetoho ea liphatsa tsa lefutso tsa likokoanyana tse nyane.
Sequencing ea Amplicon qalong e ne e sebelisetsoa ho bona sebopeho sa sechaba sa mobu, semela, kapa likokoana-hloko tsa mala a liphoofolo, tse ka sebelisoang ho sekaseka tšebelisano lipakeng tsa likokoana-hloko le liphoofolo le limela. Mokhoa oa ho latellana ha li-amplicon o thehiloe ho thekenoloji ea Next-generation Sequencing, e nang le ts'ebetso e phahameng ea ho latellana 'me e ka etsa tlhahlobo e bohareng ea palo e kholo ea lisampole.
Sehlopha se thehile mokhoa o sebetsang oa ho bona khafetsa phetoho ea liphatsa tsa lefutso ka tatellano ea amplicon. Maqhubu a L925I le T929V ho VGSC a amanang le pyrethroid resistance a ile a hlokomeloa thutong ena, e ka fanang ka lintlha tsa motheo bakeng sa taolo ea khanyetso ea B. tabaci.
Patlisiso ena e fana ka mokhoa o sebetsang hantle le o ts'epahalang oa ho lemoha khafetsa ea phetoho ea liphatsa tsa lefutso ho likokoanyana tse nyane mme e thusa ho nts'etsopele ea taolo ea likokoanyana tšimong. Patlisiso e phatlalalitsoe koranteng ea setsebi sa thuto ea entomology Pest Management Science (impact factor 3.75), Q1 ea Chinese Academy of Sciences. Mongoli oa pele oa thesis ke Wei Yiyun, mofuputsi oa postdoctoral Lab. Motlatsi oa mofuputsi Wang Ran, Dr. Qu Cheng, le Dr. Guan Fang oa Nanjing Agricultural University ba ile ba kenya letsoho karolong e 'ngoe ea mosebetsi. Mofuputsi Luo Chen ke sengoli se tsamaisanang le pampiri.
Wei, Y., Guan, F., Wang, R., Qu, C. and Luo, C. (2021), Sequencing ea Amplicon e lemoha liphetoho tse amanang le ho hanyetsa pyrethroid ho Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag
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